Jul 12, 2014, 3:47 PM
News Code: 2721027
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Iran makes new drug, gene carrier

Tehran, July 12, IRNA - A team of researchers from Tarbiat Modarres University has produced a new carrier for drug and gene delivery.

Plasmid DNA (pDNA) was entrapped in an aqueous compartment separated from the external aqueous medium by a phospholipid bilayer.

The researchers encapsulated pDNA without using multivalent cations and with 98 percent efficiency into noncationic lipid bilayer vesicles.

Multivalent cations are used out of the human bodies because of their toxicity.

These liposomes, unlike cationic liposomes, were nontoxic. The obtained liposome structure protected DNA against nuclease and was completely stable and after six months, it still kept the pDNA in its structure.

Results of the research showed that neutral liposomes originating from cell membrane are the most efficient carrier for gene delivery.

The study also indicated that in using such carriers, which are the most similar synthetic structures to viruses, their inability in electrostatic interaction with DNA would not be an obstacle for entrapping nucleic acids.

Nucleic acids are polymeric macromolecules, or large biological molecules, essential for all known forms of life. Nucleic acids include DNA.

A liposome is an artificially-prepared spherical vesicle composed of a lipid bilayer. A liposome encapsulates a region of aqueous solution inside a hydrophobic membrane; dissolved hydrophilic solutes cannot readily pass through the lipids.

Liposomes are used as models for artificial cells and can also be designed to deliver drugs and gene.

Reihaneh Ramezani, Majid Sadeqizadeh, Mehrdad Behmanesh and Saman Hossein-Khani were researchers of the study.